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CAS No 7240-90-6 , 5-Bromo-4-chloro-3-indolyl-beta-D-galactoside

  • Name: 5-Bromo-4-chloro-3-indolyl-beta-D-galactoside
  • Synonyms: 5-Bromo-4-chloro-3-indolyl-beta-D-galactoside;X-Gal;
  • CAS Registry Number:
  • Melting Point: 230 ºC (LIT.)
  • Density: 1.882g/cm3
  • Refractive index: 1.731
  • Alpha: -64 º (C=1, H2O/DMF (1/1))
  • Safety Statements: S24/25
  • Hazard Symbols: Xi
  • EINECS: 230-640-8
  • Molecular Weight: 408.63
  • InChI: InChI=1/C14H15BrClNO6/c15-5-1-2-6-9(10(5)16)7(3-17-6)22-14-13(21)12(20)11(19)8(4-18)23-14/h1-3,8,11-14,17-21H,4H2/t8-,11+,12+,13-,14-/m1/s1
  • Risk Statements: S24/25
  • Molecular Formula: C14H15BrClNO6
  • Molecular Structure:CAS No:7240-90-6 5-Bromo-4-chloro-3-indolyl-beta-D-galactoside
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7240-90-6 5-bromo-4-chloroindol-3-yl-β-D-galactopyranoside

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7240-90-6 X-Gal

  • X-Gal
  • Germany AppliChem mbH null
  • Tel: +49 6151 93 57 0
  • Fax: +49 6151 93 57 11
  • Address: Ottoweg 4 D-64291 Darmstadt null,nullGermany
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7240-90-6 5-BROMO-4-CHLORO-3-INDOLYL-?-DGALACTOPYRANOSIDE, > 98%

  • 5-BROMO-4-CHLORO-3-INDOLYL-?-DGALACTOPYRANOSIDE, > 98%
  • United States MP Biomedicals LLC [Manufacturer]
  • Tel: 877 672 2436
  • Fax: 949 859 5010
  • Address: 15 Morgan Irvine CA92618 USA ,nullUnited States
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7240-90-6 5-BROMO-4-CHLORO-3-INDOLYL

  • United States AMRESCO Inc. [Manufacturer]
  • Tel: 440-349-1313
  • Fax: 440-349-1182
  • Address: AMRESCO Inc.
    30175 Solon Industrial Parkway
    Solon, OH 44139 null,nullUnited States
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7240-90-6 5-bromo-4-chloroindol-3-yl-β-D-galactopyranoside

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7240-90-6 5-bromo-4-chloroindol-3-yl-β-D-galactopyranoside

  • China Yacoo chemical Reagent Co.,Ltd [Manufacturers]
  • Tel: +86-512-62956016ext802
  • Fax: +86-512-62956015
  • Address: A3 209 bio-bay No.218 Xinghu road suzhou industiral park Suzhou,JiangsuChina
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7240-90-6 5-bromo-4-chloroindol-3-yl-β-D-galactopyranoside

  • Austria Loba Feinchemie AG [Manufacturers]
  • Tel: ++43 (0)2232 77391-0 ++43 (0)2232 / 77391-21
  • Fax: ++43 (0)2232 76677
  • Address: Fehrgasse 7, 2401 Fischamend, Austria Fischamend,FischamendAustria
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7240-90-6 5-bromo-4-chloroindol-3-yl-β-D-galactopyranoside

  • United Kingdom Glycosynth Limited [Manufacturers]
  • Tel: 44 1925 575075
  • Fax: +44 (0) 1925 575121
  • Address: 14 Craven Court, Winwick Quay Warrington, Cheshire WA2 8QU, England Warrington,nullUnited Kingdom
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7240-90-6 5-bromo-4-chloroindol-3-yl-β-D-galactopyranoside

  • United States NetQem [Importer/Exporter]
  • Tel: +1-919-3047991
  • Fax: +1-919-3046567
  • Address: P.O. Box 12091, Research Triangle Park, NC 27709 Research Triangle Park,NCUnited States
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7240-90-6 5-bromo-4-chloroindol-3-yl-β-D-galactopyranoside

  • China Beijing Seajet Scientific [Manufacturers]
  • Tel: 86-10-88598795/7838/9569
  • Fax: +86-10-88595011
  • Address: Flat.15B, Block A.For order or inquiry please contact: Beijing, P.R.China 100089 Beijing,BeijingChina
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References of 5-Bromo-4-chloro-3-indolyl-beta-D-galactoside
Title: X-gal
CAS Registry Number: 7240-90-6
CAS Name: 5-Bromo-4-chloro-1H-indol-3-yl b-D-galactopyranoside
Molecular Formula: C14H15BrClNO6
Molecular Weight: 408.63
Percent Composition: C 41.15%, H 3.70%, Br 19.55%, Cl 8.68%, N 3.43%, O 23.49%
Literature References: Chromogenic substrate of the lacZ gene product, b-galactosidase; produces a rich blue color when cleaved by the enzyme. Prepn: J. P. Horwitz et al., J. Med. Chem. 7, 574 (1964). Colorimetric reaction in tissue to localize b-galactosidase: B. Pearson et al., Lab. Invest. 12, 1249 (1963); Z. Lojda, Histochemie 23, 266 (1970). Protocol for blue-white a-complementation screening: J. Sambrook et al., Molecular Cloning: A Laboratory Manual (Cold Spring Harbor, New York, 2nd ed., 1989) pp 1.7-1.9, 1.85-1.87. Optimization of detection of exogenous bacterial lacZ expression: D. J. Weiss et al., Hum. Gene Ther. 8, 1545 (1997); of fixative conditions in X-gal staining: W. Ma et al., J. Histochem. Cytochem. 50, 1421 (2002); of blue-white screening procedure: A. L. Sherwood, BioTechniques 34, 644 (2003).
Properties: Amorphous solid from methanol, mp 237-239° (dec). [a]D24 -69° (c = 1.0 in 50% DMF). Sol in DMF, DMSO.
Melting point: mp 237-239° (dec)
Optical Rotation: [a]D24 -69° (c = 1.0 in 50% DMF)
Use: In histochemical staining, in blue-white selection of bacterial colonies with lacZ gene activity during cloning experiments.