Chondroitinase B

Catalyzed Reaction: Chondroitinase B cleaves, via an elimination mechanism, polysaccharide chains containing 1-4 linkages between hexosamines and iduronic acid residues in dermatan sulfate (chondroitin B). The reaction yields oligosaccharide products (mainly disaccharides) containing unsaturated uronic acids which can be detected by UV spectroscopy at 232 nm.
Substrate Specificity: Dermatan sulfate (chondroitin sulfate B).
Properties:
Molecular weight: 55kDa
Isoelectric point: 9.4 - 9.6
pH optimum for activity: 7 - 8
pH range for activity: 5 - 10
Optimal temperature range: 20 ℃ - 37 ℃
Purity: ≥ 90% by reversed-phase HPLC analysis.
Standard: 1 IU and 5 IU.
Specific Activity: Greater than/equal to 200 IU/mg (substrate: dermatan sulfate). One international unit (IU) of chondroitinase B is defined as the amount of an enzyme that will liberate 1.0 μmole unsaturated oligosaccharides from dermatan sulfate per minute at 30 ℃ and pH 8.0.
Stability: 12 months froze at– 20 ℃ in aqueous buffers containing sodium phosphate and sucrose 5%.

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